nw-03: Write Results - Deep-Sea Isopod ND1
Info
Module: writing | Biology | L3 | PASS
Data
- B. jamesi: 27 tandem ND1 copies vs 1 in relatives
- 340-fold upregulation during starvation (FDR < 0.001)
- Zebrafish: 37% longer survival (28 vs 20.5 days, P=0.003)
- ATP: 72% vs 41% baseline after 14-day starvation (P < 0.001)
Output
We sequenced the genome of the deep-sea supergiant isopod Bathynomus jamesi and identified a gene encoding NADH dehydrogenase subunit 1 (ND1) whose closest phylogenetic relatives are bacterial, consistent with acquisition through ancient horizontal gene transfer. In the B. jamesi genome, ND1 is present in 27 tandem copies, contrasting with the single-copy state in shallow-water isopod relatives. Under starvation, ND1 transcription is upregulated 340-fold (RNA-seq, FDR < 0.001). ND1-expressing zebrafish exhibited median survival of 28 days compared with 20.5 days in controls, a 37% extension (P = 0.003, log-rank test, n = 40 per group). After 14 days starvation, ND1 zebrafish maintained ATP at 72% of baseline versus 41% in controls (P < 0.001).